We provide comprehensive analysis of microbiome involved in critical environmental biotechnology, such as bioremediation and wastewater treatment. Specialize in dechlorination, methanogenesis and nitrogen removal prcoess.
Dechlorination
Microbial reductive dechlorination (also known as, organohalide respiration) is the primary method for remediation of aquifers contaminated with hazardous chlorinated solvents. The organohalide-respiring bacteria (OHRB) conserve energy from the organohalide respiration process that uses chlorinated solvents as terminal electron acceptors and molecular hydrogen or low-molecular-weight compounds as electron donors.
Methanogenesis
The methanogenic archaea (methanogens) are a group of strict anaerobes that use a limited number of substrates, such as H2/CO2 (hydrogenotrophic methanogens), acetate (acetotrophic methanogens), and methyl group-containing compounds (methylotrophic methanogens)
Nitrogen removal
The deammonification process combining partial nitritation (PN) and anaerobic ammonium oxidation (anammox) has been considered as a viable option for energy-efficient wastewater treatment
Analysis item
Organohalide respiring bacteria (OHRB):
- Functional genes quantification (qPCR method): tceA, bvcA and vcrA
- OHRB quantification (qPCR method): Dehalococcoides 16S rRNA gene
- OHRB quantification (HOPE method): Dehalobacter, Desulfitobacterium, Geobacter, Dehalococcoides (subgroup- Pinellas, Victoria and Cornell)
Nitrifiers and anammox bacteria
- Functional genes quantification (qPCR method): AOB amoA, AOA amoA, Comammox amoA, Anammox hzsA, Nitrospria nxrB, nirK, nosZ
- AOB, NOB and anammox bacteria quantification (HOPE method): Nitrosomonas, Nitrosospira, Nitrobacter, Nitrospira(I and II subgroup), Brocadia, Kuenenia, Anammoxoglobus, Jettenia, Scalindua
Methanogen
4 orders and 22 genera of Methanogen
Molecular biological analysis
- Diesel-degrading Bacteria (HOPE method): Methylobacterium, Sphingomonas, Acidomonas methanolica, Strenotrophomonas, Rhodococcus, Pseudomonas
- Dioxins-degrading bacteria (HOPE method): Micrococcu, Pseudoxanthomonas, Xanthomonas,Mesorhizobium, Rhizobium,
- Total bacteria quantification (qPCR method)
- Microbial identification (cloning method)
Other analysis
- 16S rDNA metagenomics
- Bioinformatics analysis
- Plate count
- Methane, ethene, volatile acid quantification
- Ammonium, nitrite and nitrate quantification
- soil moisture content
Interested?
Please contact Dr. Chen for more information or customized services at weiuie.chen@gmail.com
MFT-LAB 